AustralieFrV1, Main, Exploration, bibRecord, 001594

The conserved apicomplexan Aurora kinase TgArk3 is involved in endodyogeny, duplication rate and parasite virulence

Identifieur interne : 001594 ( Main/Exploration ); précédent : 001593; suivant : 001595

The conserved apicomplexan Aurora kinase TgArk3 is involved in endodyogeny, duplication rate and parasite virulence

Auteurs : Laurence Berry [France] ; Chun-Ti Chen [États-Unis] ; Luc Reininger [France] ; Teresa G. Carvalho ; Hiba El Hajj [Liban] ; Juliette Morlon-Guyot [France] ; Yann Bordat [France] ; Maryse Lebrun [France] ; Marc-Jan Gubbels [États-Unis] ; Christian Doerig ; Wassim Daher [France]

Source :

Cellular microbiology [ 1462-5814 ] ; 2016.

RBID : PMC:4961599

Descripteurs français

KwdFr :
- Animaux, Aurora kinases (physiologie), Femelle, Interactions hôte-parasite, Protéines de protozoaire (physiologie), Souris, Toxoplasma (enzymologie), Toxoplasma (physiologie), Toxoplasma (ultrastructure), Toxoplasmose (parasitologie), Transport de protéines, Virulence.
MESH :
- enzymologie : Toxoplasma.
- parasitologie : Toxoplasmose.
- physiologie : Aurora kinases, Protéines de protozoaire, Toxoplasma.
- ultrastructure : Animaux, Femelle, Interactions hôte-parasite, Souris, Toxoplasma, Transport de protéines, Virulence.

English descriptors

KwdEn :
- Animals, Aurora Kinases (physiology), Female, Host-Parasite Interactions, Mice, Protein Transport, Protozoan Proteins (physiology), Toxoplasma (enzymology), Toxoplasma (physiology), Toxoplasma (ultrastructure), Toxoplasmosis (parasitology), Virulence.
MESH :
- chemical , physiology : Aurora Kinases, Protozoan Proteins.
- enzymology : Toxoplasma.
- parasitology : Toxoplasmosis.
- physiology : Toxoplasma.
- ultrastructure : Toxoplasma.
- Animals, Female, Host-Parasite Interactions, Mice, Protein Transport, Virulence.

Abstract

Aurora kinases are eukaryotic serine/threonine protein kinases that regulate key events associated with chromatin condensation, centrosome and spindle function, and cytokinesis. Elucidating the roles of Aurora kinases in apicomplexan parasites is crucial to understand the cell cycle control during Plasmodium schizogony or Toxoplasma endodyogeny. Here, we report on the localization of two previously uncharacterized Toxoplasma Aurora-related kinases (Ark2 and Ark3) in tachyzoites and of the uncharacterized Ark3 orthologue in Plasmodium falciparum erythrocytic stages. In T. gondii, we show that TgArk2 and TgArk3 concentrate at specific sub-cellular structures linked to parasite division: the mitotic spindle and intranuclear mitotic structures (TgArk2), and the outer core of the centrosome and the budding daughter cells cytoskeleton (TgArk3). By tagging the endogenous PfArk3 gene with the green fluorescent protein (GFP) in live parasites, we show that PfArk3 protein expression peaks late in schizogony and localizes at the periphery of budding schizonts. Disruption of the TgArk2 gene reveals no essential function for tachyzoite propagation in vitro, which is surprising giving that the P. falciparum and P. berghei orthologues are essential for erythrocyte schizogony. In contrast, knock-down of TgArk3 protein results in pronounced defects in parasite division and a major growth deficiency. TgArk3-depleted parasites display several defects, such as reduced parasite growth rate, delayed egress and parasite duplication, defect in rosette formation, reduced parasite size and invasion efficiency and lack of virulence in mice. Our study provides new insights into cell cycle control in Toxoplasma and malaria parasites, and highlights Aurora kinase 3 as potential drug target.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961599

DOI: 10.1111/cmi.12571
PubMed: 26833682
PubMed Central: 4961599

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The conserved apicomplexan Aurora kinase TgArk3 is involved in endodyogeny, duplication rate and parasite virulence</title>
<author><name sortKey="Berry, Laurence" sort="Berry, Laurence" uniqKey="Berry L" first="Laurence" last="Berry">Laurence Berry</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier</wicri:regionArea>
<placeName><region type="region">Occitanie (région administrative)</region>
<region type="old region">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Chun Ti" sort="Chen, Chun Ti" uniqKey="Chen C" first="Chun-Ti" last="Chen">Chun-Ti Chen</name>
<affiliation wicri:level="2"><nlm:aff id="A3">Department of Biology, Boston College, Chestnut Hill, MA 02467, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biology, Boston College, Chestnut Hill, MA 02467</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Reininger, Luc" sort="Reininger, Luc" uniqKey="Reininger L" first="Luc" last="Reininger">Luc Reininger</name>
<affiliation wicri:level="3"><nlm:aff id="A5">Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, INSERM U1016, Institut Cochin, Paris, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, INSERM U1016, Institut Cochin, Paris</wicri:regionArea>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Carvalho, Teresa G" sort="Carvalho, Teresa G" uniqKey="Carvalho T" first="Teresa G." last="Carvalho">Teresa G. Carvalho</name>
<affiliation><nlm:aff id="A2">Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Victoria, Australia, 3800</nlm:aff>
<wicri:noCountry code="subfield">3800</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="El Hajj, Hiba" sort="El Hajj, Hiba" uniqKey="El Hajj H" first="Hiba" last="El Hajj">Hiba El Hajj</name>
<affiliation wicri:level="1"><nlm:aff id="A4">Department of Internal Medicine and Experimental Pathology, Immunology and Microbiology, American University of Beirut, Beirut 1107 2020, Lebanon</nlm:aff>
<country xml:lang="fr">Liban</country>
<wicri:regionArea>Department of Internal Medicine and Experimental Pathology, Immunology and Microbiology, American University of Beirut, Beirut 1107 2020</wicri:regionArea>
<wicri:noRegion>Beirut 1107 2020</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Morlon Guyot, Juliette" sort="Morlon Guyot, Juliette" uniqKey="Morlon Guyot J" first="Juliette" last="Morlon-Guyot">Juliette Morlon-Guyot</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier</wicri:regionArea>
<placeName><region type="region">Occitanie (région administrative)</region>
<region type="old region">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bordat, Yann" sort="Bordat, Yann" uniqKey="Bordat Y" first="Yann" last="Bordat">Yann Bordat</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier</wicri:regionArea>
<placeName><region type="region">Occitanie (région administrative)</region>
<region type="old region">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lebrun, Maryse" sort="Lebrun, Maryse" uniqKey="Lebrun M" first="Maryse" last="Lebrun">Maryse Lebrun</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier</wicri:regionArea>
<placeName><region type="region">Occitanie (région administrative)</region>
<region type="old region">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gubbels, Marc Jan" sort="Gubbels, Marc Jan" uniqKey="Gubbels M" first="Marc-Jan" last="Gubbels">Marc-Jan Gubbels</name>
<affiliation wicri:level="2"><nlm:aff id="A3">Department of Biology, Boston College, Chestnut Hill, MA 02467, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biology, Boston College, Chestnut Hill, MA 02467</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Doerig, Christian" sort="Doerig, Christian" uniqKey="Doerig C" first="Christian" last="Doerig">Christian Doerig</name>
<affiliation><nlm:aff id="A2">Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Victoria, Australia, 3800</nlm:aff>
<wicri:noCountry code="subfield">3800</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Daher, Wassim" sort="Daher, Wassim" uniqKey="Daher W" first="Wassim" last="Daher">Wassim Daher</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier</wicri:regionArea>
<placeName><region type="region">Occitanie (région administrative)</region>
<region type="old region">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">26833682</idno>
<idno type="pmc">4961599</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961599</idno>
<idno type="RBID">PMC:4961599</idno>
<idno type="doi">10.1111/cmi.12571</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">001D63</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001D63</idno>
<idno type="wicri:Area/Pmc/Curation">001C19</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001C19</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000544</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000544</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">001A70</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001A70</idno>
<idno type="wicri:Area/PubMed/Curation">001A46</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001A46</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001A46</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001A46</idno>
<idno type="wicri:Area/Ncbi/Merge">003085</idno>
<idno type="wicri:Area/Ncbi/Curation">003085</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">003085</idno>
<idno type="wicri:doubleKey">1462-5814:2016:Berry L:the:conserved:apicomplexan</idno>
<idno type="wicri:Area/Main/Merge">001589</idno>
<idno type="wicri:Area/Main/Curation">001594</idno>
<idno type="wicri:Area/Main/Exploration">001594</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The conserved apicomplexan Aurora kinase TgArk3 is involved in endodyogeny, duplication rate and parasite virulence</title>
<author><name sortKey="Berry, Laurence" sort="Berry, Laurence" uniqKey="Berry L" first="Laurence" last="Berry">Laurence Berry</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier</wicri:regionArea>
<placeName><region type="region">Occitanie (région administrative)</region>
<region type="old region">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Chun Ti" sort="Chen, Chun Ti" uniqKey="Chen C" first="Chun-Ti" last="Chen">Chun-Ti Chen</name>
<affiliation wicri:level="2"><nlm:aff id="A3">Department of Biology, Boston College, Chestnut Hill, MA 02467, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biology, Boston College, Chestnut Hill, MA 02467</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Reininger, Luc" sort="Reininger, Luc" uniqKey="Reininger L" first="Luc" last="Reininger">Luc Reininger</name>
<affiliation wicri:level="3"><nlm:aff id="A5">Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, INSERM U1016, Institut Cochin, Paris, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, INSERM U1016, Institut Cochin, Paris</wicri:regionArea>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Carvalho, Teresa G" sort="Carvalho, Teresa G" uniqKey="Carvalho T" first="Teresa G." last="Carvalho">Teresa G. Carvalho</name>
<affiliation><nlm:aff id="A2">Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Victoria, Australia, 3800</nlm:aff>
<wicri:noCountry code="subfield">3800</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="El Hajj, Hiba" sort="El Hajj, Hiba" uniqKey="El Hajj H" first="Hiba" last="El Hajj">Hiba El Hajj</name>
<affiliation wicri:level="1"><nlm:aff id="A4">Department of Internal Medicine and Experimental Pathology, Immunology and Microbiology, American University of Beirut, Beirut 1107 2020, Lebanon</nlm:aff>
<country xml:lang="fr">Liban</country>
<wicri:regionArea>Department of Internal Medicine and Experimental Pathology, Immunology and Microbiology, American University of Beirut, Beirut 1107 2020</wicri:regionArea>
<wicri:noRegion>Beirut 1107 2020</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Morlon Guyot, Juliette" sort="Morlon Guyot, Juliette" uniqKey="Morlon Guyot J" first="Juliette" last="Morlon-Guyot">Juliette Morlon-Guyot</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier</wicri:regionArea>
<placeName><region type="region">Occitanie (région administrative)</region>
<region type="old region">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bordat, Yann" sort="Bordat, Yann" uniqKey="Bordat Y" first="Yann" last="Bordat">Yann Bordat</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier</wicri:regionArea>
<placeName><region type="region">Occitanie (région administrative)</region>
<region type="old region">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lebrun, Maryse" sort="Lebrun, Maryse" uniqKey="Lebrun M" first="Maryse" last="Lebrun">Maryse Lebrun</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier</wicri:regionArea>
<placeName><region type="region">Occitanie (région administrative)</region>
<region type="old region">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gubbels, Marc Jan" sort="Gubbels, Marc Jan" uniqKey="Gubbels M" first="Marc-Jan" last="Gubbels">Marc-Jan Gubbels</name>
<affiliation wicri:level="2"><nlm:aff id="A3">Department of Biology, Boston College, Chestnut Hill, MA 02467, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biology, Boston College, Chestnut Hill, MA 02467</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Doerig, Christian" sort="Doerig, Christian" uniqKey="Doerig C" first="Christian" last="Doerig">Christian Doerig</name>
<affiliation><nlm:aff id="A2">Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Victoria, Australia, 3800</nlm:aff>
<wicri:noCountry code="subfield">3800</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Daher, Wassim" sort="Daher, Wassim" uniqKey="Daher W" first="Wassim" last="Daher">Wassim Daher</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Dynamique des Interactions Membranaires Normales et Pathologiques, UMR5235 CNRS, Université Montpellier, Montpellier</wicri:regionArea>
<placeName><region type="region">Occitanie (région administrative)</region>
<region type="old region">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Cellular microbiology</title>
<idno type="ISSN">1462-5814</idno>
<idno type="eISSN">1462-5822</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Aurora Kinases (physiology)</term>
<term>Female</term>
<term>Host-Parasite Interactions</term>
<term>Mice</term>
<term>Protein Transport</term>
<term>Protozoan Proteins (physiology)</term>
<term>Toxoplasma (enzymology)</term>
<term>Toxoplasma (physiology)</term>
<term>Toxoplasma (ultrastructure)</term>
<term>Toxoplasmosis (parasitology)</term>
<term>Virulence</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Aurora kinases (physiologie)</term>
<term>Femelle</term>
<term>Interactions hôte-parasite</term>
<term>Protéines de protozoaire (physiologie)</term>
<term>Souris</term>
<term>Toxoplasma (enzymologie)</term>
<term>Toxoplasma (physiologie)</term>
<term>Toxoplasma (ultrastructure)</term>
<term>Toxoplasmose (parasitologie)</term>
<term>Transport de protéines</term>
<term>Virulence</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Aurora Kinases</term>
<term>Protozoan Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Toxoplasma</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Toxoplasma</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Toxoplasmose</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Toxoplasmosis</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Aurora kinases</term>
<term>Protéines de protozoaire</term>
<term>Toxoplasma</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Toxoplasma</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Toxoplasma</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Female</term>
<term>Host-Parasite Interactions</term>
<term>Mice</term>
<term>Protein Transport</term>
<term>Virulence</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Animaux</term>
<term>Femelle</term>
<term>Interactions hôte-parasite</term>
<term>Souris</term>
<term>Toxoplasma</term>
<term>Transport de protéines</term>
<term>Virulence</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">Aurora kinases are eukaryotic serine/threonine protein kinases that regulate key events associated with chromatin condensation, centrosome and spindle function, and cytokinesis. Elucidating the roles of Aurora kinases in apicomplexan parasites is crucial to understand the cell cycle control during <italic>Plasmodium</italic>
 schizogony or <italic>Toxoplasma</italic>
 endodyogeny. Here, we report on the localization of two previously uncharacterized <italic>Toxoplasma</italic>
 Aurora-related kinases (Ark2 and Ark3) in tachyzoites and of the uncharacterized Ark3 orthologue in <italic>Plasmodium falciparum</italic>
 erythrocytic stages. In <italic>T. gondii</italic>
, we show that TgArk2 and TgArk3 concentrate at specific sub-cellular structures linked to parasite division: the mitotic spindle and intranuclear mitotic structures (TgArk2), and the outer core of the centrosome and the budding daughter cells cytoskeleton (TgArk3). By tagging the endogenous <italic>PfArk3</italic>
 gene with the green fluorescent protein (GFP) in live parasites, we show that PfArk3 protein expression peaks late in schizogony and localizes at the periphery of budding schizonts. Disruption of the <italic>TgArk2</italic>
 gene reveals no essential function for tachyzoite propagation <italic>in vitro</italic>
, which is surprising giving that the <italic>P. falciparum</italic>
 and <italic>P. berghei</italic>
 orthologues are essential for erythrocyte schizogony. In contrast, knock-down of TgArk3 protein results in pronounced defects in parasite division and a major growth deficiency. TgArk3-depleted parasites display several defects, such as reduced parasite growth rate, delayed egress and parasite duplication, defect in rosette formation, reduced parasite size and invasion efficiency and lack of virulence in mice. Our study provides new insights into cell cycle control in <italic>Toxoplasma</italic>
 and malaria parasites, and highlights Aurora kinase 3 as potential drug target.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
<li>Liban</li>
<li>États-Unis</li>
</country>
<region><li>Languedoc-Roussillon</li>
<li>Massachusetts</li>
<li>Occitanie (région administrative)</li>
<li>Île-de-France</li>
</region>
<settlement><li>Montpellier</li>
<li>Paris</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Carvalho, Teresa G" sort="Carvalho, Teresa G" uniqKey="Carvalho T" first="Teresa G." last="Carvalho">Teresa G. Carvalho</name>
<name sortKey="Doerig, Christian" sort="Doerig, Christian" uniqKey="Doerig C" first="Christian" last="Doerig">Christian Doerig</name>
</noCountry>
<country name="France"><region name="Occitanie (région administrative)"><name sortKey="Berry, Laurence" sort="Berry, Laurence" uniqKey="Berry L" first="Laurence" last="Berry">Laurence Berry</name>
</region>
<name sortKey="Bordat, Yann" sort="Bordat, Yann" uniqKey="Bordat Y" first="Yann" last="Bordat">Yann Bordat</name>
<name sortKey="Daher, Wassim" sort="Daher, Wassim" uniqKey="Daher W" first="Wassim" last="Daher">Wassim Daher</name>
<name sortKey="Lebrun, Maryse" sort="Lebrun, Maryse" uniqKey="Lebrun M" first="Maryse" last="Lebrun">Maryse Lebrun</name>
<name sortKey="Morlon Guyot, Juliette" sort="Morlon Guyot, Juliette" uniqKey="Morlon Guyot J" first="Juliette" last="Morlon-Guyot">Juliette Morlon-Guyot</name>
<name sortKey="Reininger, Luc" sort="Reininger, Luc" uniqKey="Reininger L" first="Luc" last="Reininger">Luc Reininger</name>
</country>
<country name="États-Unis"><region name="Massachusetts"><name sortKey="Chen, Chun Ti" sort="Chen, Chun Ti" uniqKey="Chen C" first="Chun-Ti" last="Chen">Chun-Ti Chen</name>
</region>
<name sortKey="Gubbels, Marc Jan" sort="Gubbels, Marc Jan" uniqKey="Gubbels M" first="Marc-Jan" last="Gubbels">Marc-Jan Gubbels</name>
</country>
<country name="Liban"><noRegion><name sortKey="El Hajj, Hiba" sort="El Hajj, Hiba" uniqKey="El Hajj H" first="Hiba" last="El Hajj">Hiba El Hajj</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001594 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001594 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:4961599
   |texte=   The conserved apicomplexan Aurora kinase TgArk3 is involved in endodyogeny, duplication rate and parasite virulence
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:26833682" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a AustralieFrV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Dec 5 10:43:12 2017. Site generation: Tue Mar 5 14:07:20 2024

Serveur d'exploration sur les relations entre la France et l'Australie

The conserved apicomplexan Aurora kinase TgArk3 is involved in endodyogeny, duplication rate and parasite virulence

The conserved apicomplexan Aurora kinase TgArk3 is involved in endodyogeny, duplication rate and parasite virulence

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur les relations entre la France et l'Australie
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.